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Order Name LAB1365 HPV DNA DETECTION with GENOTYPING, PCR

Important Note

HPV testing may be performed on the same ThinPrep sample submitted for cytologic evaluation. This test can only be performed on cervical samples. To order HPV testing on a ThinPrep Pap test, circle yes for HPV Detection, in the ThinPrep area on the laboratory requisition form or in Epic under Pap Test (LAB4), question #4 under the prompt “Please Specify HPV Testing”.  If you have questions, please call Cytopathology at (802)-847-5121.

HPV Testing:  Anal Pap tests have not been approved by the FDA for HPV testing therefore, our laboratory will not perform HPV testing on this type of sample.  There is no FDA-approved HPV test for anal or oral samples, therefore we do not perform this testing and will not forward to a reference lab.

Outside clients submit a manual order.

Vaginal thinprep requests for HPV Genotyping will be sent to Mayo Clinic Laboratories.

Method

RT-PCR

CPT Code(s)

Description CPT Code
HPV Detection, High Risk Types                               87624

 

Specimen Information

Container Specimen Temperature Collect Vol Submit Vol Min Vol Stability
ThinPrep Vial Cervical Ambient 4 mL 4 mL 1 mL 30 days

Collect specimen in ThinPrep PreservCyt (Pap) vials with broom-type or cytobrush/spatula collection devices according to the manufacturer’s instructions. Specimen is stable for 30 days at 2-30° C. This testing may be ordered as reflex testing to a diagnosis of Atypical Squamous Cells- Undetermined Significance (ASC-US). HPV testing may also be ordered up front, regardless of the diagnosis of the current ThinPrep cytology result. 

Vaginal samples are NOT acceptable, vaginal high risk HPV samples will be sent to Mayo Medical Laboratory for testing.

Reference Range

Negative for HPV. No DNA detected from HPV types 16,18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68.

Instrumentation

Roche Cobas 6800

 

Performing Location

University of Vermont Medical Center

Test Schedule / Analytical Time / Test Priority

Monday – Friday / 1 day / Not available STAT

Section

Chemistry

Is the UVMMC lab NY State Certified to perform this testing?  Yes/No

Yes