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HelpOrder Name FRFIT Rabies Antibody Endpoint
Reporting Name
Rabies RFFIT Endpoint, SPerforming Laboratory
RFFIT/K-State Rabies Laboratory Manhattan/K-State Innovation CenterSpecimen Type
SerumSpecimen Required
Specimen Required
Specimen Type: Serum
Container/Tube: Red
Specimen Volume: 2 mL
Collection Instructions: Draw blood in a plain red-top tube(s), Spin down and send 2 mL of serum refrigerated in a plastic vial.
Note: 1. Serum gel tube is acceptable, but must be poured off into plastic vial.
2. Collection date is required.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 14 days | |
| Frozen | 60 days |
Reference Values
Reportable range is 0.1 to 15.0 IU/mL
Less than 0.1 IU/mL: Below detection limit
In humans a results of 0.5 IU/mL or higher is considered an acceptable response to rabies vaccination according to the World Health Organization (WHO) guidelines; see WHO and Advisory Committee on Immunization Practices documents for additional guidance.
Day(s) Performed
Monday, Wednesday, Thursday
CPT Code Information
86382
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| FRFIT | Rabies RFFIT Endpoint, S | 6524-3 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| Z2071 | Rabies Titer-Response | 6524-3 |
Interpretation
Quantitative results. For those who want to know their exact titer between the reportable range.
RFFIT stands for Rapid Fluorescent Foci Inhibition Test. It is a serum neutralization (inhibition) test, which means it measures the ability of rabies specific antibodies to neutralize rabies virus and prevent the virus from infecting cells. The antibodies are called rabies virus neutralizing antibodies (RVNA).
Method Description
In RFFIT, your serum (the non-cellular portion of your blood) is first diluted fivefold (1part serum in 4 parts diluent). Further (serial fivefold) dilutions are performed, each of which contain less and less of your serum. These serum dilutions are mixed with a standard amount of live rabies virus and incubated. Whatever RVNA is present will neutralize the virus. Next, some tissue culture cells are added and the serum/virus/cells are incubated together. Whatever rabies virus is left (i.e., that which has not been neutralized by the antibody in your serum), will infect the cells and this can be seen under the microscope through the use of specific staining. Calculation of the endpoint titer is made from the percent of virus infected cells observed on the slide.
Report Available
3 to 5 weeksReject Due To
| Hemolysis | Mild OK; Gross reject |
| Lipemia | Mild OK; Gross reject |
| Icterus | NA |
| Other | NA |
NY State Approved
YesMethod Name
Serum Neutralization Fluorescent Antibody